POSTDOC AND PHD POSITIONS IN STRUCTURAL BIOLOGY OF WNT SIGNALING

Typ práce: hlavní pracovní poměr

Description

CEITEC is a scientific centre in the fields of life sciences, advanced materials and technologies whose aim is to establish itself as a recognized centre for basic as well as applied research. CEITEC offers a state-of-the-art infrastructure and great conditions to employ excellent researchers.

Our institute values diversity and promotes an inclusive working environment with the aim to support the best scientists. CEITEC MU is a proud holder of the HR Excellence in Research Award by the European Commission. (http://muni.ceitec.cz/en/hr-strategy-hrs4r/)

Research Group: Protein-DNA interactions

Research Group Leader: Konstantinos Tripsianes, Ph.D.

Project information:

We apply structural biology methods in order to gain a mechanistic view of CK1? action in the Wnt signalling pathways. CK1? represents an attractive therapeutic target but currently two key steps in the CK1?-mediated Wnt signal transduction are unclear: (PostDoc Project) how CK1? gets activated and/or engages target proteins in response to Wnt signal and (PhD project) how CK1? phosphorylates its key substrate DVL and what are the consequences of DVL phosphorylation.

Our preliminary data suggest that we can efficiently apply methods of integrated structural biology to (i) probe the DVL conformational landscape using in vitro and in vivo FRET sensors coupled to SAXS and CryoEM, (ii) understand the (auto)phosphorylation regulatory mechanisms of CK1?, (iii) analyse by NMR the functional consequences of DVL phosphorylation and (iv) monitor DVL phosphorylation by real-time NMR under controlled cellular conditions.

Both positions are part of a multidisciplinary project that combines (i) cellular and molecular biology, (ii) proteomic analysis, (iii) biochemistry and structural biology, and received generous funding in a very competitive grant scheme.

CEITEC is a vibrant work place with excellent colleagues and infrastructure comparable to the best institutions in Europe for: (i) cryo-electron microscopy (Titan Krios with phase plate, Falcon3, and K2 direct electron detectors, Talos Arctica equipped with Falcon3 direct electron detector, Tecnai F20, FIB/SEM Versa3D, and vitrification robot), (ii) X-ray crystallography equipped with the BioSAXS arm on the Rigaku diffraction system, (iii) high-field NMR (500 MHz, 600 MHz, 2 × 700 MHz, 850 MHz, and 950 MHz equipped with high-sensitivity cryogenically cooled probeheads), (iv) biointeractions (SPR, ITC, DSC, MST, DSF, AUC, CD).

Requirements:

We are looking for highly motivated researchers with a strong background or interest in structural biology. Hands-on experience with protein expression and purification is prerequisite. Experience with biomolecular NMR is a plus.

We offer:

• Salary that will be based on the candidate’s qualifications and experience.
• Background of a recognized and successful institution with supportive and international working environment
• Support with the relocation process (welcome office service)
• 6 weeks of paid holiday

Anticipated start date: Immediately or upon agreement.

Please send application (including CV, short statement of research background/interests and the contact information of at least two references) or queries by email to: kostas.tripsianes@ceitec.muni.cz

Please quote the “position” in the subject.

 Electronic application deadline is: December 24^th 2019

Kontaktní osoba:
Konstantinos Tripsianes, Ph.D.

E-mail:
kostas.tripsianes@ceitec.muni.cz

WWW:
https://www.ceitec.cz/

Telefon:
+420 54949 6607

Vloženo: 24.10.2019